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Jeong Hoon Song, M.D., Min Sun Kim, M.D.*, Byung Rim Park, M.D.*, Han Su Park**, Jeong Ryong Chae**, Hye Me Lee, M.D., Young Cheon Na, M.D. |
| 흰쥐 후지근 피판에서 허혈-재순환 손상시 pERK1/2 발현에 대한 α-lipoic acid의 효과 |
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송정훈·김민선*·박병림*·박한수* *·채정룡* *·이혜미·나영천 |
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| Abstract |
Purpose: This study was to evaluate the effect of α-lipoic acid, a potent free radical scavenger, on the expression of active form of extracellular signal-regulated kinase (pERK1/2) proteins from hindlimb muscles of rats following ischemia-reperfusion injury.
Material and methods: 64 health, 280~350 g weighted Sprague-Dawley male rats were used. In order to make a muscle flap, the gastrocnemius (GC) and soleus (SOL) muscles were dissected and elevated. The popliteal artery was occluded for 4hours and reperfused for 10 minutes, 30 minutes, 1 hour, 2 hours and 4 hours, respectively.
Results: The ischemia by occlusion of the popliteal artery itself caused a minimal change in expression of phosphorylated form of proteins observed in hindlimb muscle. In contrast, after 4 hours of ischemia, immunoreactivity for pERK1/2 in the GC muscle showed dual peaks at 10 minutes and 4 hours after reperfusion. In α-lipoic acid treated group, the expression of pERK1/2 was increased significantly compared to I/R-only group.
Conclusion: These results suggest that α-lipoic acid may protect I/R injury of the skeletal muscle through free radical scavening and activation of intracellular pERK1/2 expression |
| Key Words:
α-Lipoic acid, pERK1/2, Ischemia-reperfusion injury, Muscle flap |
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