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Journal of the Korean Society for Surgery of the Hand 2001;6(1):1-6.
Published online January 1, 2001.
Expression of Marker Gene(lacZ) Mediated with Baculoviral Vector in Achilles Tendon of Rabbit
Sun U. Song, Do Seung Kwon, Kyoung Back Choi, Mi Sook Choi, Kwan Hee Lee
배큘로바이러스 벡터를 사용한 표적 유전자의 가토 아킬레스건내의 발현
송순욱, 권도승, 최경백, 최미숙, 이관희
Abstract
Purpose
: To investigate that expression of a marker gene, lacZ, mediated with a baculoviral vector can be obtained in Achilles tendon. The ultimate goal is to find out the feasibility of utilizing a baculoviral vector for the gene therapeutic treatment of tendon injuries. Materials and Methods : A recombinant baculovirus (BV-pCMV-lacZ) containing a marker gene lacZ was produced by co-transfecting baculoviral transfer vector and genomic DNA into Sf21 insect cells. Purified recombinant baculoviruses were injected into the Achilles tendon of rabbit. The expression of β-galactosidase protein was evaluated by immunohistochemical staining at 1, 3, and 7 days after injection of the baculoviruses. Results : The expression of β-galactosidase from BVpCMV-lacZ baculovirus was evaluated by infection of Saos-2 cells, which are very susceptible to baculoviruses, and subsequent western blot analysis. The expression of β-galactosidase in the tendon was detected at 1, 3, and 7 days after injection of the baculoviruses. The level of β-galactosidase expression was highest at 1 day post injection and decreased at 3 and 7 days after injection. Conclusion : The results showed the feasibility of a baculoviral vector being utilized as a gene delivery tool for the gene therapeutic treatment of tendon injuries.
 
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