Expression of Marker Gene(lacZ) Mediated with Baculoviral Vector in Achilles Tendon of Rabbit |
Sun U. Song, Do Seung Kwon, Kyoung Back Choi, Mi Sook Choi, Kwan Hee Lee |
배큘로바이러스 벡터를 사용한 표적 유전자의 가토 아킬레스건내의 발현 |
송순욱, 권도승, 최경백, 최미숙, 이관희 |
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Abstract |
Purpose : To investigate that expression of a marker gene, lacZ, mediated with a baculoviral vector can be obtained in Achilles tendon. The ultimate goal is to find out the feasibility of utilizing a baculoviral vector for the gene therapeutic treatment of tendon injuries. Materials and Methods : A recombinant baculovirus (BV-pCMV-lacZ) containing a marker gene lacZ was produced by co-transfecting baculoviral transfer vector and genomic DNA into Sf21 insect cells. Purified recombinant baculoviruses were injected into the Achilles tendon of rabbit. The expression of β-galactosidase protein was evaluated by immunohistochemical staining at 1, 3, and 7 days after injection of the baculoviruses. Results : The expression of β-galactosidase from BVpCMV-lacZ baculovirus was evaluated by infection of Saos-2 cells, which are very susceptible to baculoviruses, and subsequent western blot analysis. The expression of β-galactosidase in the tendon was detected at 1, 3, and 7 days after injection of the baculoviruses. The level of β-galactosidase expression was highest at 1 day post injection and decreased at 3 and 7 days after injection. Conclusion : The results showed the feasibility of a baculoviral vector being utilized as a gene delivery tool for the gene therapeutic treatment of tendon injuries. |
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