| The Change of Type I Procollagen mRNA Expression by Growth Factors in Rabbit Deep Flexor Tendon Cell |
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Ho-Jung Kang, Woo-Suk Lee, Sang-Jin Shin, Soo Bong Hahn, Eung-Shick Kang |
| 성장인자에 의한 토끼의 심부 굴곡건 세포 제1형 교원질의 mRNA 표현 변화 |
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강호정, 이우석, 신상진, 한수봉, 강응식 |
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| Abstract |
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- epidermal growth factor (EGF), insulin-like growth factor (IGF), platelet-derived growth factor (PDGF), and transforming growth factor (TGF) - known as affecting the metabolism of the flexor tendon in short term culture of cells isolated from rabbit deep flexor tendons. Materials and Methods : Cells were isolated from rabbit deep flexor tendons by enzymatic digestion and cultured with Dulbecco's modified eagles medium (DMEM) containing 0.5% fetal bovine serum (FBS). And then four kinds of growth factors (10ng/ml) were given into each. The control cultures were kept as plain cultures without growth factors. On Day 5, Day 10, and Day 15, the cells were collected and cell proliferation rate was carried out by cell counting with hemocytometer. The expression of typeⅠprocollagen mRNA was measured by the Northern blot analysis. Results : In proliferation assay, The 10% FBS group showed the highest cell pro-liferation. The EGF group and the TGF group showed increased cell proliferation compared to the IGF group and PDGF group (p<0.05). The IGF group and PDGF group showed significant increase in type I procollagen mRNA expression (p<0.05), but the EGF group and the TGF group showed no more expression than the control group. Conclusion : Based on this study, we conclude that the PDGF and the IGF to be the most effective growth factors in the early healing process of rabbit flexor tendon. |
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